|
| Status |
Public on Feb 22, 2012 |
| Title |
Liver GPR120KO HFD #427 |
| Sample type |
RNA |
| |
|
| Source name |
Liver GPR120KO HFD
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
genotype: GPR120 KO
treatment: high fat diet HFD
genetic background: mixed 129/Sv and C57BL/6
subject: mouse 427
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by guanidine hydrochloride-phenol-chloroform method and transcribed to cDNA.
|
| Label |
biotin
|
| Label protocol |
cDNA labeling was performed according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
The labeled cDNA were then hybridized on Affimetrix gene chip and stained.
|
| Scan protocol |
The probe arrays were scanned with the GeneChip(R) Scanner.
|
| Data processing |
The data were analyzed with R software. The MAS 5.0 normalization were performed using the justMAS function in the simpleaffy package from Bioconductor.
|
| |
|
| Submission date |
Sep 13, 2011 |
| Last update date |
Feb 22, 2012 |
| Contact name |
Akira Hirasawa |
| E-mail(s) |
akira_h@pharm.kyoto-u.ac.jp
|
| Phone |
075-753-4543
|
| Organization name |
Kyoto University Graduate School of Pharmaceutical Sciences
|
| Department |
Genomic Drug Discovery Science
|
| Street address |
46-29 Yoshida Shimoadachi-cho, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32095 |
GPR120 mediates high-fat diet induced obesity |
|
