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Sample GSM797442 Query DataSets for GSM797442
Status Public on Sep 16, 2011
Title Alternative activated macrophages (M2) rep 1
Sample type RNA
 
Source name Alternative activated macrophages (M2)
Organism Homo sapiens
Characteristics source tissue: normal donor buffy coats
source cell type: freshly isolated monocytes
cell type: macrophages
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
 
Hybridization protocol Following fragmentation, 2.7 ug of cDNA were hybridized for 16 hr at 45C on Human Genome HuGene 1.0 ST . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned were scanned using the Affymetrix GeneChip Scanner 3000.
Description Donor_1 M2_rep1
R743_N2
Data processing Expression dataset was obtained by means of Partek software version 6.3, with RMA method.
 
Submission date Sep 15, 2011
Last update date Sep 01, 2016
Contact name Massimo Locati
E-mail(s) transcriptional.profiles@humanitasresearch.it
Phone +39 02 8224 5116
Fax 02-82245101
URL http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
Organization name Istituto Clinico Humanitas
Department Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
Lab Laboratory of Leukocyte Biology
Street address Via Manzoni, 113
City Rozzano
State/province MILAN
ZIP/Postal code 20089
Country Italy
 
Platform ID GPL6244
Series (1)
GSE32164 Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7892501 4.0821
7892502 3.36487
7892503 2.55577
7892504 9.18029
7892505 2.33562
7892506 3.01572
7892507 2.67028
7892508 4.65779
7892509 11.4394
7892510 3.57679
7892511 3.22343
7892512 6.51367
7892513 4.4034
7892514 10.5798
7892515 8.44554
7892516 5.5092
7892517 4.83512
7892518 1.81379
7892519 3.80306
7892520 8.47805

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM797442_R743_N2_HuGene-1_0-st-v1_.CEL.gz 3.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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