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Sample GSM797443 Query DataSets for GSM797443
Status Public on Sep 16, 2011
Title Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 1
Sample type RNA
 
Source name Human macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors
Organism Homo sapiens
Characteristics source tissue: normal donor buffy coats
source cell type: freshly isolated monocytes
cell type: macrophages
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
 
Hybridization protocol Following fragmentation, 2.7 ug of cDNA were hybridized for 16 hr at 45C on Human Genome HuGene 1.0 ST . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned were scanned using the Affymetrix GeneChip Scanner 3000.
Description Donor_1 MycI_rep1
R743_N3
Data processing Expression dataset was obtained by means of Partek software version 6.3, with RMA method.
 
Submission date Sep 15, 2011
Last update date Sep 01, 2016
Contact name Massimo Locati
E-mail(s) transcriptional.profiles@humanitasresearch.it
Phone +39 02 8224 5116
Fax 02-82245101
URL http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
Organization name Istituto Clinico Humanitas
Department Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
Lab Laboratory of Leukocyte Biology
Street address Via Manzoni, 113
City Rozzano
State/province MILAN
ZIP/Postal code 20089
Country Italy
 
Platform ID GPL6244
Series (1)
GSE32164 Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7892501 5.89799
7892502 3.39503
7892503 2.20494
7892504 9.3099
7892505 2.09217
7892506 3.58349
7892507 3.51987
7892508 4.25086
7892509 11.1652
7892510 3.28477
7892511 3.10022
7892512 6.39169
7892513 3.77826
7892514 10.2717
7892515 8.49301
7892516 4.72487
7892517 4.80633
7892518 1.87587
7892519 3.10839
7892520 8.4039

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM797443_R743_N3_HuGene-1_0-st-v1_.CEL.gz 3.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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