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Sample GSM797446 Query DataSets for GSM797446
Status Public on Sep 16, 2011
Title Macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors, rep 2
Sample type RNA
 
Source name Human macrophages where c-Myc activation was blocked by using c-Myc chemical inibitors
Organism Homo sapiens
Characteristics source tissue: normal donor buffy coats
source cell type: freshly isolated monocytes
cell type: macrophages
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
 
Hybridization protocol Following fragmentation, 2.7 ug of cDNA were hybridized for 16 hr at 45C on Human Genome HuGene 1.0 ST . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned were scanned using the Affymetrix GeneChip Scanner 3000.
Description Donor_2 MycI_rep2
R743_N6
Data processing Expression dataset was obtained by means of Partek software version 6.3, with RMA method.
 
Submission date Sep 15, 2011
Last update date Sep 01, 2016
Contact name Massimo Locati
E-mail(s) transcriptional.profiles@humanitasresearch.it
Phone +39 02 8224 5116
Fax 02-82245101
URL http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
Organization name Istituto Clinico Humanitas
Department Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
Lab Laboratory of Leukocyte Biology
Street address Via Manzoni, 113
City Rozzano
State/province MILAN
ZIP/Postal code 20089
Country Italy
 
Platform ID GPL6244
Series (1)
GSE32164 Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7892501 4.93224
7892502 3.14721
7892503 2.91194
7892504 9.75123
7892505 2.59014
7892506 3.64926
7892507 2.9354
7892508 4.25464
7892509 11.0156
7892510 3.88494
7892511 2.44406
7892512 6.77233
7892513 3.52115
7892514 9.14606
7892515 8.29342
7892516 4.75013
7892517 5.56049
7892518 2.17866
7892519 3.08916
7892520 8.56924

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM797446_R743_N6_HuGene-1_0-st-v1_.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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