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Sample GSM797447 Query DataSets for GSM797447
Status Public on Sep 16, 2011
Title Resting fully differentiated macrophages (7 days of culture), rep 3
Sample type RNA
 
Source name Resting fully differentiated human macrophages (7 days of culture)
Organism Homo sapiens
Characteristics source tissue: normal donor buffy coats
source cell type: freshly isolated monocytes
cell type: macrophages
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA.
 
Hybridization protocol Following fragmentation, 2.7 ug of cDNA were hybridized for 16 hr at 45C on Human Genome HuGene 1.0 ST . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned were scanned using the Affymetrix GeneChip Scanner 3000.
Description Donor_3 M0_rep3
R743_N7
Data processing Expression dataset was obtained by means of Partek software version 6.3, with RMA method.
 
Submission date Sep 15, 2011
Last update date Sep 01, 2016
Contact name Massimo Locati
E-mail(s) transcriptional.profiles@humanitasresearch.it
Phone +39 02 8224 5116
Fax 02-82245101
URL http://www.humanitas.it/hur/cms/english/activities/lab/leukocyte_biology/groupleader.html
Organization name Istituto Clinico Humanitas
Department Department of Translational Medicine of the University of Milan Faculty of Medicine, IRCCS Istituto Clinico Humanitas
Lab Laboratory of Leukocyte Biology
Street address Via Manzoni, 113
City Rozzano
State/province MILAN
ZIP/Postal code 20089
Country Italy
 
Platform ID GPL6244
Series (1)
GSE32164 Expression data from macrophage maturation and polarization c-Myc in alternative macrophage activation experiments
Relations
Reanalyzed by GSE86357

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7892501 5.32305
7892502 3.21528
7892503 2.79388
7892504 9.55252
7892505 2.22804
7892506 3.03773
7892507 2.92499
7892508 5.13512
7892509 11.2617
7892510 4.45742
7892511 3.19011
7892512 6.73921
7892513 3.38251
7892514 10.2842
7892515 8.5035
7892516 5.93882
7892517 5.77318
7892518 2.60702
7892519 3.27968
7892520 9.24756

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM797447_R743_N7_HuGene-1_0-st-v1_.CEL.gz 3.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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