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| Status |
Public on Apr 03, 2013 |
| Title |
embryo injected with anti-mir-34LNA, kept for 7hpf, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
embryo injected with anti-mir-34LNA, kept for 7hpf
|
| Organism |
Danio rerio |
| Characteristics |
genotype: wild type
tissue: anti-mir-34LNA injected embryos, dechorionated
embryo age: 7 hour post fertilization
|
| Treatment protocol |
Embryos were collected at one cell stage. Embryos were injected with anti-mir-34LNA of mockLNA at the 1-2 cell stage and were harvetyes at the specified time points of 1hpf, 7hpf and 24hpf. The embryos were also monitoredunder the dissecting microscope for any developmental abnormalities of phenotypic defects. Selected embryos were transferred to a fresh petriplate. The injected embryos at 7hpf and 24hpf were dechorionated manually, rinsed with distilled water and placed on ice in the Trizol solution (GibcoBRL).
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| Growth protocol |
1-2 cell stage embryos were collected for microinjection. Following injections the embryos were aged at 30C and were lated collected at the different time points (1hpf, 7hpf and 24hpf)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol as per the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Zebrafish embryos at 7 hour post fertilization(hpf) indicating transcript pool during maternal to zygotic transcription
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| Data processing |
Initial QC and processing was done using Affymetrix GeneChip Operating Software(GCOS) to obtain the raw signal intensities. The data were analyzed with Avadis using the default analysis settings and quantile normalization as normalization method afer being log transformed.
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| |
|
| Submission date |
Sep 23, 2011 |
| Last update date |
Apr 03, 2013 |
| Contact name |
Kartik Soni |
| E-mail(s) |
kartik@igib.in
|
| Organization name |
INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY
|
| Department |
FGU
|
| Lab |
MOL. BIO
|
| Street address |
Mathura Road
|
| City |
NEW DELHI |
| State/province |
NA |
| ZIP/Postal code |
110028 |
| Country |
India |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE32360 |
Expression data from early Zebrafish embryos after knockdown of mir-34 |
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