|
| Status |
Public on Aug 24, 2012 |
| Title |
Kidney2 |
| Sample type |
RNA |
| |
|
| Source name |
Adult zebrafish kidney
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: tg(jam1a: EGFP)
tissue: whole kidney tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIzol reagent (Invitrogen) and purified on RNeasy Mini kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNAs were generated from total RNAs using GeneChip 3' IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Adult kidney tissue isolated from Tg(jam1a:EGFP) zebrafish
|
| Data processing |
Expression values were calculated by RMA algorithm using the affy package of Bioconductor (http://www.bioconductor.org/).
|
| |
|
| Submission date |
Sep 24, 2011 |
| Last update date |
Aug 24, 2012 |
| Contact name |
Isao Kobayashi |
| Organization name |
Keio University
|
| Department |
Cell Differentiation
|
| Street address |
35 Shinanoma-chi
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE32363 |
Gene expression profile of zebrafish renal collecting ducts |
|
