|
| Status |
Public on Feb 07, 2012 |
| Title |
splenic innate response activator (IRA) cells 4 |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
gender: female
strain: C57BL/6
cell type: innate response activator (IRA) cells
cell signature: CD19+ MHC Class II+ CD93+ IgM+ CD23- CD43+ VLA-4hi
treatment: LPS 0.4mg/kg, i.p., 4 days
|
| Treatment protocol |
Up to 10 animals were pooled per sample. Animals were steady state or received LPS 0.4mg/kg, i.p. for 4 consecutive days.
|
| Growth protocol |
6-8 wk old C57BL/6 mice, on regular chow diet.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell were isolated by fluorescent activated cell sorting on a BD FACS Aria II directly into RLT lysis buffer and total RNA was extracted using the Qiagen RNA easy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Illumina BeadStation (Illumina)
|
| |
|
| Hybridization protocol |
Illumina BeadStation (Illumina)
|
| Scan protocol |
Illumina BeadStation (Illumina)
|
| Description |
IRA_4
|
| Data processing |
Gene Pattern 3.3.2, Build ID 9695, IlluminaExpressionFileCreator (background substraction: true), IlluminaNormalizer (normalization method: quantile)
|
| |
|
| Submission date |
Sep 26, 2011 |
| Last update date |
Feb 07, 2012 |
| Contact name |
Martin Etzrodt |
| E-mail(s) |
ETZRODT.MARTIN@MGH.HARVARD.EDU
|
| Phone |
(617) 643-0500
|
| Fax |
(617) 643-6133
|
| URL |
http://csb.mgh.harvard.edu/pittet
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Systems Biology
|
| Lab |
Pittet Lab
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE32372 |
Innate response activator B cells are sentinels that guard against polymicrobial sepsis |
|
