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Sample GSM801446 Query DataSets for GSM801446
Status Public on Feb 07, 2012
Title splenic marginal zone B cells 3
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics tissue: spleen
gender: female
strain: C57BL/6
cell type: marginal zone B cells
cell signature: CD19+ MHC Class II+ CD93- IgM+ CD23- CD21hi
treatment: LPS 0.4mg/kg, i.p., 4 days
Treatment protocol Up to 10 animals were pooled per sample. Animals were steady state or received LPS 0.4mg/kg, i.p. for 4 consecutive days.
Growth protocol 6-8 wk old C57BL/6 mice, on regular chow diet.
Extracted molecule total RNA
Extraction protocol Cell were isolated by fluorescent activated cell sorting on a BD FACS Aria II directly into RLT lysis buffer and total RNA was extracted using the Qiagen RNA easy mini kit (Qiagen).
Label biotin
Label protocol Illumina BeadStation (Illumina)
 
Hybridization protocol Illumina BeadStation (Illumina)
Scan protocol Illumina BeadStation (Illumina)
Description MZ_3
Data processing Gene Pattern 3.3.2, Build ID 9695, IlluminaExpressionFileCreator (background substraction: true), IlluminaNormalizer (normalization method: quantile)
 
Submission date Sep 26, 2011
Last update date Feb 07, 2012
Contact name Martin Etzrodt
E-mail(s) ETZRODT.MARTIN@MGH.HARVARD.EDU
Phone (617) 643-0500
Fax (617) 643-6133
URL http://csb.mgh.harvard.edu/pittet
Organization name Massachusetts General Hospital
Department Center for Systems Biology
Lab Pittet Lab
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL6885
Series (1)
GSE32372 Innate response activator B cells are sentinels that guard against polymicrobial sepsis

Data table header descriptions
ID_REF
VALUE AVG Signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 -6.154761905 0.8659148
ILMN_1212612 5.565 0.2130326
ILMN_1212619 -13.51952381 0.9974937
ILMN_1212628 7.756190476 0.1578947
ILMN_1212632 -1.978380952 0.6253133
ILMN_1212636 696.952381 0
ILMN_1212637 45.99142857 0.001253133
ILMN_1212645 13.46357143 0.06265664
ILMN_1212648 105.1428571 0
ILMN_1212653 3.43952381 0.3120301
ILMN_1212672 9.541904762 0.112782
ILMN_1212682 -4.71952381 0.7932331
ILMN_1212683 -3.246190476 0.7042606
ILMN_1212685 0.042985714 0.4937343
ILMN_1212692 1.222809524 0.4273183
ILMN_1212693 11.00428571 0.09273183
ILMN_1212695 4.56952381 0.2481203
ILMN_1212698 -8.868095238 0.933584
ILMN_1212702 323.9666667 0
ILMN_1212703 8.545238095 0.1353384

Total number of rows: 25697

Table truncated, full table size 830 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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