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Sample GSM801618 Query DataSets for GSM801618
Status Public on Apr 01, 2012
Title 66977_ro
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics background strain: C57BL/6
tissue: tumor
genotype/variation: mutALK
Treatment protocol none
Growth protocol Mice transgenic for neural crest specific expression of MYCN, mutated ALK or both were monitored until tumor was palpable and sacrificed afterwards.
Extracted molecule total RNA
Extraction protocol RNA was extracted from tumors or control tissue using the QIAGEN Rneasy mini kit.
Label biotin
Label protocol Biotin labelling was performed according the manufacturer's recommendations (Affymetrix).
 
Hybridization protocol Hybridisation to mouse 430_2 arrays was performed according to the manufacturer's recommendations (Affymetrix).
Scan protocol Scanning of chips was performed using a GeneArray scanner (Agilent).
Description tumor driven by mutALK
Data processing Signal intensities were RMA normalized using PARTEK Genomics Suite, with the .CEL files as input.
 
Submission date Sep 26, 2011
Last update date Apr 13, 2012
Contact name Alexander Schramm
E-mail(s) alexander.schramm@uk-essen.de
Organization name University Hospital Essen
Department Childrens Hospital
Lab Hematology/Oncology Lab
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL1261
Series (1)
GSE32386 Expression profiling of murine neuroblastoma in transgenic mice

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity

Data table
ID_REF VALUE
1415670_at 9.14915
1415671_at 11.4755
1415672_at 11.1777
1415673_at 7.56212
1415674_a_at 10.2312
1415675_at 9.41423
1415676_a_at 11.2525
1415677_at 9.5549
1415678_at 10.9829
1415679_at 10.899
1415680_at 8.73638
1415681_at 9.78195
1415682_at 7.93879
1415683_at 10.6858
1415684_at 8.78944
1415685_at 9.3069
1415686_at 10.4469
1415687_a_at 12.3704
1415688_at 10.8007
1415689_s_at 8.82871

Total number of rows: 45101

Table truncated, full table size 850 Kbytes.




Supplementary file Size Download File type/resource
GSM801618.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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