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Sample GSM801621 Query DataSets for GSM801621
Status Public on Apr 01, 2012
Title 74128_wlr
Sample type RNA
 
Source name tumor
Organism Mus musculus
Characteristics background strain: C57BL/6
tissue: tumor
genotype/variation: MYCN
Treatment protocol none
Growth protocol Mice transgenic for neural crest specific expression of MYCN, mutated ALK or both were monitored until tumor was palpable and sacrificed afterwards.
Extracted molecule total RNA
Extraction protocol RNA was extracted from tumors or control tissue using the QIAGEN Rneasy mini kit.
Label biotin
Label protocol Biotin labelling was performed according the manufacturer's recommendations (Affymetrix).
 
Hybridization protocol Hybridisation to mouse 430_2 arrays was performed according to the manufacturer's recommendations (Affymetrix).
Scan protocol Scanning of chips was performed using a GeneArray scanner (Agilent).
Description tumor driven by MYCN
Data processing Signal intensities were RMA normalized using PARTEK Genomics Suite, with the .CEL files as input.
 
Submission date Sep 26, 2011
Last update date Apr 13, 2012
Contact name Alexander Schramm
E-mail(s) alexander.schramm@uk-essen.de
Organization name University Hospital Essen
Department Childrens Hospital
Lab Hematology/Oncology Lab
Street address Hufelandstr. 55
City Essen
ZIP/Postal code 45122
Country Germany
 
Platform ID GPL1261
Series (1)
GSE32386 Expression profiling of murine neuroblastoma in transgenic mice

Data table header descriptions
ID_REF
VALUE RMA-normalized signal intensity

Data table
ID_REF VALUE
1415670_at 9.37813
1415671_at 10.4943
1415672_at 11.2597
1415673_at 8.4424
1415674_a_at 10.0406
1415675_at 9.11929
1415676_a_at 11.5055
1415677_at 8.28011
1415678_at 10.4519
1415679_at 10.5421
1415680_at 9.77151
1415681_at 10.2294
1415682_at 8.67269
1415683_at 11.2193
1415684_at 8.72041
1415685_at 9.8951
1415686_at 10.0958
1415687_a_at 12.0812
1415688_at 10.817
1415689_s_at 9.18704

Total number of rows: 45101

Table truncated, full table size 849 Kbytes.




Supplementary file Size Download File type/resource
GSM801621.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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