|
| Status |
Public on Dec 01, 2012 |
| Title |
Tumor #1, GFP-negative/mCherry+ sorted cells |
| Sample type |
RNA |
| |
|
| Source name |
myf5-GFP negative/mylz2-mCherry+ cell subfraction, Tumor #1
|
| Organism |
Danio rerio |
| Characteristics |
strain: transgenic, CG1-strain (4 times outcrossed)
sample type: FACS sorted ERMS cells
cell type: myf5-GFP negative/mylz2-mCherry+ cell subfraction, Tumor #1
disease state: embryonal rhabdomyosarcoma
|
| Growth protocol |
ERMS tumors were transplanted into recipient animals, allowed to engraft, and then isolated at >20 days post-transplantation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
NuGEN pico label
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA System
|
| |
|
| Hybridization protocol |
Washing and staining were performed on the GeneChip® Fluidics Station
|
| Scan protocol |
The GeneChip® Scanner 3000 was used for scanning the arrays
|
| Description |
Gene expression data from FACS sorted ERMS cell subpopulations
|
| Data processing |
Samples were imported into D-Chip, normalized against an invariant set, and log2 transformed
|
| |
|
| Submission date |
Sep 27, 2011 |
| Last update date |
Dec 01, 2012 |
| Contact name |
David M Langenau |
| E-mail(s) |
dlangenau@partners.org
|
| Phone |
617643-6508
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Pathology
|
| Lab |
Langenau Lab
|
| Street address |
149 13th Street #6012
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02180 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE32425 |
Expression profile of zebrafish embryonal rhabdomyosarcoma |
|
