|
Status |
Public on Dec 01, 2012 |
Title |
Tumor #2, GFP-negative/mCherry-negative, Double Negative (DN) sorted cells |
Sample type |
RNA |
|
|
Source name |
myf5-GFP negative/mylz2-mCherry negative cell subfraction, Tumor #2
|
Organism |
Danio rerio |
Characteristics |
strain: transgenic, CG1-strain (4 times outcrossed) sample type: FACS sorted ERMS cells cell type: myf5-GFP negative/mylz2-mCherry negative cell subfraction, Tumor #2 disease state: embryonal rhabdomyosarcoma
|
Growth protocol |
ERMS tumors were transplanted into recipient animals, allowed to engraft, and then isolated at >20 days post-transplantation.
|
Extracted molecule |
total RNA |
Extraction protocol |
NuGEN pico label
|
Label |
biotin
|
Label protocol |
Ovation Pico WTA System
|
|
|
Hybridization protocol |
Washing and staining were performed on the GeneChip® Fluidics Station
|
Scan protocol |
The GeneChip® Scanner 3000 was used for scanning the arrays
|
Description |
Gene expression data from FACS sorted ERMS cell subpopulations
|
Data processing |
Samples were imported into D-Chip, normalized against an invariant set, and log2 transformed
|
|
|
Submission date |
Sep 27, 2011 |
Last update date |
Dec 01, 2012 |
Contact name |
David M Langenau |
E-mail(s) |
dlangenau@partners.org
|
Phone |
617643-6508
|
Organization name |
Massachusetts General Hospital
|
Department |
Molecular Pathology
|
Lab |
Langenau Lab
|
Street address |
149 13th Street #6012
|
City |
Charlestown |
State/province |
MA |
ZIP/Postal code |
02180 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE32425 |
Expression profile of zebrafish embryonal rhabdomyosarcoma |
|