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Sample GSM802476 Query DataSets for GSM802476
Status Public on Dec 01, 2012
Title Tumor #2, GFP-negative/mCherry-negative, Double Negative (DN) sorted cells
Sample type RNA
 
Source name myf5-GFP negative/mylz2-mCherry negative cell subfraction, Tumor #2
Organism Danio rerio
Characteristics strain: transgenic, CG1-strain (4 times outcrossed)
sample type: FACS sorted ERMS cells
cell type: myf5-GFP negative/mylz2-mCherry negative cell subfraction, Tumor #2
disease state: embryonal rhabdomyosarcoma
Growth protocol ERMS tumors were transplanted into recipient animals, allowed to engraft, and then isolated at >20 days post-transplantation.
Extracted molecule total RNA
Extraction protocol NuGEN pico label
Label biotin
Label protocol Ovation Pico WTA System
 
Hybridization protocol Washing and staining were performed on the GeneChip® Fluidics Station
Scan protocol The GeneChip® Scanner 3000 was used for scanning the arrays
Description Gene expression data from FACS sorted ERMS cell subpopulations
Data processing Samples were imported into D-Chip, normalized against an invariant set, and log2 transformed
 
Submission date Sep 27, 2011
Last update date Dec 01, 2012
Contact name David M Langenau
E-mail(s) dlangenau@partners.org
Phone 617643-6508
Organization name Massachusetts General Hospital
Department Molecular Pathology
Lab Langenau Lab
Street address 149 13th Street #6012
City Charlestown
State/province MA
ZIP/Postal code 02180
Country USA
 
Platform ID GPL1319
Series (1)
GSE32425 Expression profile of zebrafish embryonal rhabdomyosarcoma

Data table header descriptions
ID_REF
VALUE log2 transformed in Dchip

Data table
ID_REF VALUE
Dr.1372.1.S1_at 6.42
Dr.354.1.S1_at 5.6
Dr.1109.1.S1_at 10.97
Dr.1411.1.S1_at 12.05
Dr.1434.1.S1_at 3.84
Dr.11308.1.S1_at 9.88
Dr.296.1.S1_at 6.94
Dr.1383.1.S1_at 10.33
Dr.1368.1.S1_a_at 10.74
Dr.261.1.S1_at 11.2
Dr.1246.1.S1_at 4.31
Dr.24922.1.S1_at 12.07
Dr.3499.2.S1_a_at 4.47
Dr.20008.1.S1_at 10.09
Dr.268.1.S1_at 4.57
Dr.1320.1.S1_at 9.75
Dr.6496.1.S1_at 11.24
Dr.25155.1.S1_s_at 11.88
Dr.8153.2.S1_a_at 9.8
Dr.8153.1.S1_at 4.33

Total number of rows: 15502

Table truncated, full table size 327 Kbytes.




Supplementary file Size Download File type/resource
GSM802476_Tumor__2-G-R-_DN_.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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