|
| Status |
Public on Sep 27, 2011 |
| Title |
Individual 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Human platelets, hyperreactivity
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: platelets
disease state: healthy
platelet reactivity to 1.5 um epinephrine: hyper
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from lymphocyte-depleted platelets using Trizol following the manufacturer's protocol.
|
| Label |
Hy3
|
| Label protocol |
RNA was labeled with the miRCURY LNA™ microRNA Power Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Common reference
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: platelets
disease state: healthy
composition: mixture of all the samples pooled together
sample type: reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from lymphocyte-depleted platelets using Trizol following the manufacturer's protocol.
|
| Label |
Hy5
|
| Label protocol |
RNA was labeled with the miRCURY LNA™ microRNA Power Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Samples were hybridzied using Tecan hybridization stations in which the hybridization and washing steps are fully automated.
|
| Scan protocol |
Samples were scanned on Agilent G2505B Microarray Scanners operated in an ozone-free environment to avoid bleaching of the fluorescent labels. Image analysis was done with ImaGene.
|
| Description |
DH002
|
| Data processing |
Data were background subtracted and LOWESS normalized using the Bioconductor LIMMA package.
|
| |
|
| Submission date |
Sep 27, 2011 |
| Last update date |
Sep 27, 2011 |
| Contact name |
Leonard C. Edelstein |
| Organization name |
Thomas Jefferson University
|
| Department |
Medicine/Hematology
|
| Lab |
Paul F. Bray
|
| Street address |
1015 Walnut St.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE27917 |
Platelet microRNA-mRNA Co-Expression Profiles Correlate with Platelet Reactivity |
|
