|
Status |
Public on Sep 27, 2011 |
Title |
Individual 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Human platelets, hyperreactivity
|
Organism |
Homo sapiens |
Characteristics |
tissue: platelets disease state: healthy platelet reactivity to 1.5 um epinephrine: hyper
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from lymphocyte-depleted platelets using Trizol following the manufacturer's protocol.
|
Label |
Hy3
|
Label protocol |
RNA was labeled with the miRCURY LNA™ microRNA Power Labeling Kit.
|
|
|
Channel 2 |
Source name |
Common reference
|
Organism |
Homo sapiens |
Characteristics |
tissue: platelets disease state: healthy composition: mixture of all the samples pooled together sample type: reference
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from lymphocyte-depleted platelets using Trizol following the manufacturer's protocol.
|
Label |
Hy5
|
Label protocol |
RNA was labeled with the miRCURY LNA™ microRNA Power Labeling Kit.
|
|
|
|
Hybridization protocol |
Samples were hybridzied using Tecan hybridization stations in which the hybridization and washing steps are fully automated.
|
Scan protocol |
Samples were scanned on Agilent G2505B Microarray Scanners operated in an ozone-free environment to avoid bleaching of the fluorescent labels. Image analysis was done with ImaGene.
|
Description |
DH002
|
Data processing |
Data were background subtracted and LOWESS normalized using the Bioconductor LIMMA package.
|
|
|
Submission date |
Sep 27, 2011 |
Last update date |
Sep 27, 2011 |
Contact name |
Leonard C. Edelstein |
Organization name |
Thomas Jefferson University
|
Department |
Medicine/Hematology
|
Lab |
Paul F. Bray
|
Street address |
1015 Walnut St.
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19107 |
Country |
USA |
|
|
Platform ID |
GPL7723 |
Series (1) |
GSE27917 |
Platelet microRNA-mRNA Co-Expression Profiles Correlate with Platelet Reactivity |
|