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Sample GSM803602 Query DataSets for GSM803602
Status Public on Sep 30, 2011
Title patient 12, arm treated with pimecrolimus
Sample type RNA
 
Source name patient 12, left arm treated with pimecrolimus until day 22
Organism Homo sapiens
Characteristics atopic dermatitis: mild-to-moderate AD
tissue: skin biopsy
peasi reduction [%]: 60
Treatment protocol Treated twice daily for 3 weeks on 1 upper limb with 1% pimecrolimus cream and on the other upper limb with 0.1% betamethasone valerate cream in a double blind manner.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, approximately 10 ug of cRNA were hybridized for 17 hr at 45C on Gene Chip HG U133 plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneArray Scanner GSC3000
Description Gene expression data from human skin biopsy sample from atopic dermatitis patient treated with pimecrolimus
12_PC
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Sep 29, 2011
Last update date Oct 02, 2011
Contact name Andreas Scherer
E-mail(s) Andreas.Scherer@Spheromics.com
Organization name Spheromics
Street address Pajutie 3A
City Kontiolahti
ZIP/Postal code 81100
Country Finland
 
Platform ID GPL570
Series (1)
GSE32473 Gene expression is differently affected by pimecrolimus and betamethasone in lesional skin of atopic dermatitis.

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 459.6 P
AFFX-BioB-M_at 755.9 P
AFFX-BioB-3_at 495.4 P
AFFX-BioC-5_at 1251.6 P
AFFX-BioC-3_at 1093.1 P
AFFX-BioDn-5_at 3570.4 P
AFFX-BioDn-3_at 5270.8 P
AFFX-CreX-5_at 14609.5 P
AFFX-CreX-3_at 16490.7 P
AFFX-DapX-5_at 7.5 A
AFFX-DapX-M_at 10.3 A
AFFX-DapX-3_at 1.2 A
AFFX-LysX-5_at 3.4 A
AFFX-LysX-M_at 9.6 A
AFFX-LysX-3_at 2.7 A
AFFX-PheX-5_at 8.4 A
AFFX-PheX-M_at 1.1 A
AFFX-PheX-3_at 7.3 A
AFFX-ThrX-5_at 2.6 A
AFFX-ThrX-M_at 3.3 A

Total number of rows: 54675

Table truncated, full table size 948 Kbytes.




Supplementary file Size Download File type/resource
GSM803602_Patient_12_Pimecrolimus.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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