|
| Status |
Public on Sep 30, 2011 |
| Title |
patient 15, baseline |
| Sample type |
RNA |
| |
|
| Source name |
patient 15, baseline
|
| Organism |
Homo sapiens |
| Characteristics |
atopic dermatitis: mild-to-moderate AD
tissue: skin biopsy
peasi reduction [%]: 0
|
| Treatment protocol |
Treated twice daily for 3 weeks on 1 upper limb with 1% pimecrolimus cream and on the other upper limb with 0.1% betamethasone valerate cream in a double blind manner.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, approximately 10 ug of cRNA were hybridized for 17 hr at 45C on Gene Chip HG U133 plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner GSC3000
|
| Description |
Gene expression data from human skin biopsy sample from atopic dermatitis patient
15_Bsl
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Sep 29, 2011 |
| Last update date |
Oct 02, 2011 |
| Contact name |
Andreas Scherer |
| E-mail(s) |
Andreas.Scherer@Spheromics.com
|
| Organization name |
Spheromics
|
| Street address |
Pajutie 3A
|
| City |
Kontiolahti |
| ZIP/Postal code |
81100 |
| Country |
Finland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE32473 |
Gene expression is differently affected by pimecrolimus and betamethasone in lesional skin of atopic dermatitis. |
|
