|
Status |
Public on Aug 02, 2012 |
Title |
Y16wks6_grp1a_RA5LU |
Sample type |
RNA |
|
|
Source name |
Kidney from lupus NZB/W mouse (16 weeks old)
|
Organism |
Mus musculus |
Characteristics |
disease status: Kidney from prenephritic mouse tissue: Whole kidney
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Trizol and manufacturer's instructions
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
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|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
Description |
Gene expression data from NZB/W prenephritic kidney (16 weeks old, non-proteinuric)
|
Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization; and Combat for batch correction within the NZB/W mouse model and the NZW/BXSB mouse model.
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|
|
Submission date |
Oct 03, 2011 |
Last update date |
Aug 15, 2013 |
Contact name |
Celine C Berthier |
Organization name |
University of Michigan
|
Street address |
1150 W. Medical Center Dr, MSRB2
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL7546 |
Series (3) |
GSE32583 |
Expression data from lupus NZB/W, NZM2410, NZW/BXSB mouse kidneys prenephritic and nephritic. |
GSE32592 |
Human and mouse lupus nephritis cross-species transcriptional analysis |
GSE50038 |
Immunopathogenesis of renal inflammation during the progression, remission and relapse of renal disease in the NZB/W murine lupus model |
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