|
| Status |
Public on Aug 02, 2012 |
| Title |
Sick3_grp4_Claire1 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney from lupus NZB/W mouse (36 weeks old)
|
| Organism |
Mus musculus |
| Characteristics |
disease status: Kidney from prenephritic mouse (36 weeks old, proteinuric)
tissue: Whole kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol and manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Gene expression data from NZB/W nephritic kidney (36 weeks old, proteinuric)
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization; and Combat for batch correction within the NZB/W mouse model and the NZW/BXSB mouse model.
|
| |
|
| Submission date |
Oct 03, 2011 |
| Last update date |
Aug 15, 2013 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL7546 |
| Series (3) |
| GSE32583 |
Expression data from lupus NZB/W, NZM2410, NZW/BXSB mouse kidneys prenephritic and nephritic. |
| GSE32592 |
Human and mouse lupus nephritis cross-species transcriptional analysis |
| GSE50038 |
Immunopathogenesis of renal inflammation during the progression, remission and relapse of renal disease in the NZB/W murine lupus model |
|
