|
| Status |
Public on Nov 29, 2012 |
| Title |
P_H16_I9_a1474_M |
| Sample type |
RNA |
| |
|
| Source name |
Kidney
|
| Organism |
Mus musculus |
| Characteristics |
genotype (n: Control; P: Mutant): P
age induced (days): 9
age harvested (days): 16
gender (m: Male; F: Female; U: Unknown): M
litter: 5
batch: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen), followed by RNeasy plus kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cRNA was generated and labeled by incorporating biotin-16-UTP.
|
| |
|
| Hybridization protocol |
0.75 ugs of biotin-labeled cRNA was hybridized according to standard Illumina protocol.
|
| Scan protocol |
Standard Illumina protocol.
|
| Description |
Array name: X4443087015_C
Title format: Genotype_AgeHarvested_AgeInduced_AnimalNumber_Gender
Label of raw data matrix: LM5
|
| Data processing |
The data were normalized using VST transformation with lummi in R.
|
| |
|
| Submission date |
Oct 04, 2011 |
| Last update date |
Nov 29, 2012 |
| Contact name |
Luis Fernando Menezes |
| E-mail(s) |
luis.menezes@nih.gov
|
| Organization name |
National Institutes of Health
|
| Department |
NIDDK
|
| Street address |
Room 8D12A, Building 10, 10 Center Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE32586 |
Transcriptomic and metabolomic analysis of autosomal dominant polycystic kidney disease identifies HNF4α as a disease modifier |
|
