|
Status |
Public on Nov 29, 2012 |
Title |
N_H12_I7_N_P12_M_16-10_M |
Sample type |
RNA |
|
|
Source name |
Kidney
|
Organism |
Mus musculus |
Characteristics |
genotype (n: Control; P: Mutant): N age induced (days): 7 age harvested (days): 12 gender (m: Male; F: Female; U: Unknown): M litter: 16 batch: 6
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using Trizol (Invitrogen), followed by RNeasy plus kit (Qiagen)
|
Label |
biotin
|
Label protocol |
cRNA was generated and labeled by incorporating biotin-16-UTP.
|
|
|
Hybridization protocol |
0.75 ugs of biotin-labeled cRNA was hybridized according to standard Illumina protocol.
|
Scan protocol |
Standard Illumina protocol.
|
Description |
Array name: X16.10_LM8.006 Title format: Genotype_AgeHarvested_AgeInduced_AnimalNumber_Gender Label of raw data matrix: LM8
|
Data processing |
The data were normalized using VST transformation with lummi in R.
|
|
|
Submission date |
Oct 04, 2011 |
Last update date |
Nov 29, 2012 |
Contact name |
Luis Fernando Menezes |
E-mail(s) |
luis.menezes@nih.gov
|
Organization name |
National Institutes of Health
|
Department |
NIDDK
|
Street address |
Room 8D12A, Building 10, 10 Center Dr
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE32586 |
Transcriptomic and metabolomic analysis of autosomal dominant polycystic kidney disease identifies HNF4α as a disease modifier |
|