NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM813924 Query DataSets for GSM813924
Status Public on Oct 16, 2011
Title ID3 knockout mouse CD8+ T cells, pooled sample#3, exon-level
Sample type RNA
 
Source name CD8+ T cells
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: ID3 knockout
batch: Array batch 2
Extracted molecule total RNA
Extraction protocol RNEasy Micro kit (Qiagen), following manufatcturer's instructions
Label biotin
Label protocol WT expression kit (Ambion), WT Terminal Labeling Kit (Affymetrix), according to the manufatcturer's instructions
 
Hybridization protocol Samples were hybridized to WT Human Gene 1.0 ST arrays using Affymetrix hybridization kit materials, according to the manufacturrer's instructions. Sample cocktails were heated at 99°C for 5 minutes, then 45°C for 5 minutes, centrifuged at max speed for 1 minute. Eighty ul of hyb solution was transferred to each array, and hybridized 17 hours at 45°C at 60rpm. Fluidics washing protocol used was FS450_0007.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description ID3 KO- 3
Id3 KO-3_(MoGene-1_0-st-v1).CEL
mouse ID3-/- CD8+ T cells, pooled sample derived from four mice, sample #3
Data processing Data from .CEL files were imported into Partek Genomincs Suite using the RMA method. For gene level analyses, probeset level data were merged using the median probeset expression level.
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
 
Submission date Oct 11, 2011
Last update date Oct 16, 2011
Contact name Zoltan Pos
E-mail(s) pos.zoltan@outlook.com.com
Phone +(36)12102930-56435
Organization name Semmelweis University
Department Dept. of Genetics, Cell- and Immunobiology
Street address 4 Nagyvarad ter
City Budapest
ZIP/Postal code H-1089
Country Hungary
 
Platform ID GPL10740
Series (1)
GSE23568 Expression data analyzing ID3 (Inhibitor of DNA binding 3)-affected mouse T cells
Relations
Alternative to GSM813932 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE Log 2-transformed RMA signal intensities from Partek GS

Data table
ID_REF VALUE
10338001 11.3276
10338002 5.07176
10338003 8.78226
10338004 7.7463
10338005 5.07603
10338006 4.36845
10338007 4.23028
10338008 4.21696
10338009 6.90756
10338010 5.07904
10338011 4.61582
10338012 4.65361
10338013 7.38617
10338014 6.58616
10338015 7.09935
10338016 6.07999
10338017 12.4498
10338018 5.17716
10338019 4.5842
10338020 6.68611

Total number of rows: 241576

Table truncated, full table size 3984 Kbytes.




Supplementary file Size Download File type/resource
GSM813924.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap