|
| Status |
Public on Oct 03, 2012 |
| Title |
Gfi1KO_ENU_Tum-2 |
| Sample type |
RNA |
| |
|
| Source name |
thymocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Gfi1 KO
protocol: ENU transformed
|
| Treatment protocol |
Overall procedure: Gfi1f/f (WT_Tum) or Mx1Cre+Gfi1f/f (KO_Tum) or Gfi1f/f Notch tg (WT_Notch_Tum) or Mx1Cre+Gfi1f/f Notch tg (Notch_KO_Tum)Mice were injected with di-Nitriso-N-Ethylurea (ENU) and observed for emergence of mediastinal using ultarsound. Upon emergence of a growing mediastinal tumor, mice were injected with pIpC, which induces deletion of Gfi1 in MxCre+ tg mice. About 2-3 weeks after pIpC injection, tumor cells were explanted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Quiagen micro-RNA kit was used
|
| Label |
biotin
|
| Label protocol |
Target RNA was reverse transcribed into cDNA using a NuGEN Ovation Pico RNA Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
|
| |
|
| Hybridization protocol |
Samples were hybridized with [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Arrays and scanned using a GeneArray Scanner at the Génome Québec INNOVATION centre of the McGill University, Montréal, QC, Canada
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Gfi1KO_Tum-057.CEL
|
| Data processing |
Data was processed using the AltAnalyze_V.2.0.3 Win32 software
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Oct 03, 2012 |
| Contact name |
Lothar Vassen |
| E-mail(s) |
lothar.vassen@ircm.qc.ca
|
| Phone |
514-987-3351
|
| Organization name |
IRCM
|
| Department |
Hematopoiesis and Cancer
|
| Lab |
Dr. Möröy
|
| Street address |
110, avenue des Pins Ouest
|
| City |
Montréal |
| State/province |
Quebec |
| ZIP/Postal code |
H2W 1R7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE32910 |
Growth factor independence 1 (Gfi1) as a new target for leukemia therapy |
|
