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Sample GSM814742 Query DataSets for GSM814742
Status Public on Oct 03, 2012
Title WT_Notch_Tum-1
Sample type RNA
 
Source name thymocytes
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: wt
protocol: transformed by Notch1-CT and ENU
Treatment protocol Overall procedure: Gfi1f/f (WT_Tum) or Mx1Cre+Gfi1f/f (KO_Tum) or Gfi1f/f Notch tg (WT_Notch_Tum) or Mx1Cre+Gfi1f/f Notch tg (Notch_KO_Tum)Mice were injected with di-Nitriso-N-Ethylurea (ENU) and observed for emergence of mediastinal using ultarsound. Upon emergence of a growing mediastinal tumor, mice were injected with pIpC, which induces deletion of Gfi1 in MxCre+ tg mice. About 2-3 weeks after pIpC injection, tumor cells were explanted.
Extracted molecule total RNA
Extraction protocol Quiagen micro-RNA kit was used
Label biotin
Label protocol Target RNA was reverse transcribed into cDNA using a NuGEN Ovation Pico RNA Kit and in-vitro transcription was performed to generate biotin-labeled cRNA. cRNA was labeled with streptavidin-phycoerythrin.
 
Hybridization protocol Samples were hybridized with [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Arrays and scanned using a GeneArray Scanner at the Génome Québec INNOVATION centre of the McGill University, Montréal, QC, Canada
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description WT_Notch_Tum-061.CEL
Data processing Data was processed using the AltAnalyze_V.2.0.3 Win32 software
 
Submission date Oct 12, 2011
Last update date Oct 03, 2012
Contact name Lothar Vassen
E-mail(s) lothar.vassen@ircm.qc.ca
Phone 514-987-3351
Organization name IRCM
Department Hematopoiesis and Cancer
Lab Dr. Möröy
Street address 110, avenue des Pins Ouest
City Montréal
State/province Quebec
ZIP/Postal code H2W 1R7
Country Canada
 
Platform ID GPL10740
Series (1)
GSE32910 Growth factor independence 1 (Gfi1) as a new target for leukemia therapy

Data table header descriptions
ID_REF
VALUE log2 scale GC-RMA signal values

Data table
ID_REF VALUE
10344615 8.51878
10344617 3.84668
10344625 10.21181
10344626 11.70374
10344627 9.48188
10344628 11.5992
10344629 9.11726
10344630 10.95676
10344631 11.53511
10344632 11.76933
10344634 10.09049
10344636 11.06282
10344639 10.96479
10344640 12.18301
10344641 9.52682
10344642 9.05951
10344643 7.3111
10344644 8.95718
10344645 10.21953
10344646 9.2613

Total number of rows: 182058

Table truncated, full table size 3063 Kbytes.




Supplementary file Size Download File type/resource
GSM814742.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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