|
| Status |
Public on Dec 01, 2013 |
| Title |
Normal breast tissue 010 |
| Sample type |
RNA |
| |
|
| Source name |
Normal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Normal tissue
|
| Growth protocol |
Normal Human Mammary Epithelial Cells (HMEC) (Clonetics) were grown in MEGM, Mammary Epithelial Growth Medium (Clonetics) supplemented with growth factors SingleQuots (Clonetics) in absence of FBS and with 2%penicilin/streptomicine at 37ºC and 5% CO2 atmosphere and 80% humidity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell pellet using Trizol following manufacturer's recommendations
|
| Label |
Hy3
|
| Label protocol |
300ng of total RNA were treated for with Calf Intestine Phosphatase and than labeled with Hy3 fluorescent dye
|
| |
|
| Hybridization protocol |
Exiqon miRNA microarray slides containing denaturated labeled RNA in 1x hybridization buffer were hybridized at 56ºC for 16h
|
| Scan protocol |
Slides were scanned using Agilent array scanner set to 10μm wit 100PMT sensitivity in an ozone-free-environment. Fluorescence intensities on scanned images were quantified using Feature Extraction software (Agilent) using the modified Exiqon protocol
|
| Description |
Normal breast tissue
|
| Data processing |
Agilent feature extraction software (version 9.5.3) was run on all array datasets using the GE2-v5_95_Feb07 protocol. Normexp and quantiles were applied for background correction and normalization respectively.
|
| |
|
| Submission date |
Oct 12, 2011 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Beatriz Martinez |
| E-mail(s) |
bmartinez@cnio.es
|
| Organization name |
Spanish National Cancer Research Centre (CNIO)
|
| Department |
Human Cancer Genetics
|
| Street address |
Calle Melchor Fernandez Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
| GSE32922 |
Comparison between hereditary breast tumors and normal breast tissue samples. |
|
