|
| Status |
Public on Oct 13, 2011 |
| Title |
Naïve CD69+ T cells |
| Sample type |
RNA |
| |
|
| Source name |
Naïve mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: male
tissue: spleen and lymph node
cell type: CD69+ T cell fraction
recipient status: naïve
|
| Treatment protocol |
After incubation for 20-21 h, recipient CD3+CD69+ or CD3+CD69+ T cells were isolated using FACS cell sorter.
|
| Growth protocol |
T cells isolated from peripheral lymphoid tissues of recipient mice were cocultured with donor T cell-depleted splenocytes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified from isolated T cells using RNeasy Plus Mini Kit (Qiagen) according to the manufacturers’ protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX).
|
| |
|
| Hybridization protocol |
Following fragmentation, 1.5 ug of cRNA were hybridized to the Illumina MouseWG-6 Expression Beadchip according to the protocols provided by the manufacturer.
|
| Scan protocol |
Array were scanned using the Illumina Bead Array Reader Confocal Scanner.
|
| Description |
Nor CD69+ T
CD69+ T cell fraction after stimulation with donor antigen
|
| Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2009.2 (Gene Expression Module v1.5.4). Genes were filtered to include only genes with a detection p value less than 0.05 in at least 50% of samples. Out of 45,281 probes, 16,001 were thus used for further analysis after transformation of their normalized signal intensities into log2 values.
|
| |
|
| Submission date |
Oct 13, 2011 |
| Last update date |
Oct 13, 2011 |
| Contact name |
Dong-Gyun Lim |
| E-mail(s) |
dglim@nmc.or.kr
|
| Organization name |
National Medical Center, Research Institute
|
| Street address |
245 Eulji-ro, Jung-gu
|
| City |
Seoul |
| ZIP/Postal code |
100-799 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE32953 |
Gene expression profiles of T cell fraction enriched for donor antigen-reactive T cells in skin allografted recipient mice (II) |
| GSE32955 |
Gene expression profiles of T cell fraction enriched for donor antigen-reactive T cells in skin allografted recipient mice |
|
