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Status |
Public on Oct 13, 2011 |
Title |
Tolerance CD69+ T cells |
Sample type |
RNA |
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Source name |
Tolerant recipient mice, d100
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: male tissue: spleen and lymph node cell type: CD69+ T cell fraction recipient status: tolerant
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Treatment protocol |
After incubation for 20-21 h, recipient CD3+CD69+ or CD3+CD69+ T cells were isolated using FACS cell sorter.
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Growth protocol |
T cells isolated from peripheral lymphoid tissues of recipient mice were cocultured with donor T cell-depleted splenocytes.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted and purified from isolated T cells using RNeasy Plus Mini Kit (Qiagen) according to the manufacturers’ protocol.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin, TX).
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Hybridization protocol |
Following fragmentation, 1.5 ug of cRNA were hybridized to the Illumina MouseWG-6 Expression Beadchip according to the protocols provided by the manufacturer.
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Scan protocol |
Array were scanned using the Illumina Bead Array Reader Confocal Scanner.
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Description |
Tol CD69+ T CD69+ T cell fraction after stimulation with donor antigen
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Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2009.2 (Gene Expression Module v1.5.4). Genes were filtered to include only genes with a detection p value less than 0.05 in at least 50% of samples. Out of 45,281 probes, 16,001 were thus used for further analysis after transformation of their normalized signal intensities into log2 values.
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Submission date |
Oct 13, 2011 |
Last update date |
Oct 13, 2011 |
Contact name |
Dong-Gyun Lim |
E-mail(s) |
dglim@nmc.or.kr
|
Organization name |
National Medical Center, Research Institute
|
Street address |
245 Eulji-ro, Jung-gu
|
City |
Seoul |
ZIP/Postal code |
100-799 |
Country |
South Korea |
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Platform ID |
GPL6887 |
Series (2) |
GSE32953 |
Gene expression profiles of T cell fraction enriched for donor antigen-reactive T cells in skin allografted recipient mice (II) |
GSE32955 |
Gene expression profiles of T cell fraction enriched for donor antigen-reactive T cells in skin allografted recipient mice |
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