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Sample GSM818675 Query DataSets for GSM818675
Status Public on Jan 01, 2014
Title CML_3284_G1
Sample type RNA
 
Source name CML Patient: Diagnosis
Organism Homo sapiens
Characteristics source: Bone Marrow
tissue type: tumor, at diagnosis
age: 62
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
Label biotin
Label protocol Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
 
Hybridization protocol Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
Scan protocol Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm.
 
Submission date Oct 18, 2011
Last update date Jan 01, 2014
Contact name ROCIO BENITO
E-mail(s) beniroc@usal.es
Organization name centro investigacion del cancer
Lab 12
Street address Campus Unamuno s/n
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL570
Series (1)
GSE33075 Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression

Data table header descriptions
ID_REF
VALUE RMA NORMALIZED EXPRESSION VALUE

Data table
ID_REF VALUE
AFFX-BioB-5_at 5.90631
AFFX-BioB-M_at 7.210111
AFFX-BioB-3_at 6.742398
AFFX-BioC-5_at 8.356055
AFFX-BioC-3_at 8.614777
AFFX-BioDn-5_at 8.73173
AFFX-BioDn-3_at 11.00518
AFFX-CreX-5_at 11.71006
AFFX-CreX-3_at 12.24269
AFFX-DapX-5_at 3.359956
AFFX-DapX-M_at 5.037112
AFFX-DapX-3_at 6.577404
AFFX-LysX-5_at 2.94675
AFFX-LysX-M_at 3.355513
AFFX-LysX-3_at 4.478113
AFFX-PheX-5_at 3.076495
AFFX-PheX-M_at 3.916887
AFFX-PheX-3_at 5.446095
AFFX-ThrX-5_at 3.3592
AFFX-ThrX-M_at 3.221045

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM818675_SAL_00031.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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