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Sample GSM818676 Query DataSets for GSM818676
Status Public on Jan 01, 2014
Title CML_3305_H1
Sample type RNA
 
Source name CML Patient: Diagnosis
Organism Homo sapiens
Characteristics source: Bone Marrow
tissue type: tumor, at diagnosis
age: 70
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
Label biotin
Label protocol Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
 
Hybridization protocol Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
Scan protocol Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm.
 
Submission date Oct 18, 2011
Last update date Jan 01, 2014
Contact name ROCIO BENITO
E-mail(s) beniroc@usal.es
Organization name centro investigacion del cancer
Lab 12
Street address Campus Unamuno s/n
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL570
Series (1)
GSE33075 Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression

Data table header descriptions
ID_REF
VALUE RMA NORMALIZED EXPRESSION VALUE

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.665449
AFFX-BioB-M_at 7.931688
AFFX-BioB-3_at 7.422479
AFFX-BioC-5_at 8.865102
AFFX-BioC-3_at 9.154509
AFFX-BioDn-5_at 9.275821
AFFX-BioDn-3_at 11.17339
AFFX-CreX-5_at 12.0722
AFFX-CreX-3_at 12.4672
AFFX-DapX-5_at 4.156223
AFFX-DapX-M_at 6.511778
AFFX-DapX-3_at 7.613686
AFFX-LysX-5_at 2.876805
AFFX-LysX-M_at 3.776507
AFFX-LysX-3_at 5.032345
AFFX-PheX-5_at 2.722706
AFFX-PheX-M_at 4.078174
AFFX-PheX-3_at 5.973101
AFFX-ThrX-5_at 3.305882
AFFX-ThrX-M_at 3.424788

Total number of rows: 54675

Table truncated, full table size 1051 Kbytes.




Supplementary file Size Download File type/resource
GSM818676_SAL_00032.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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