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Sample GSM818678 Query DataSets for GSM818678
Status Public on Jan 01, 2014
Title CML_2623_B2
Sample type RNA
Source name CML Patient: Treated
Organism Homo sapiens
Characteristics source: Bone Marrow
tissue type: tumor, treated
age: 70
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
Label biotin
Label protocol Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
Hybridization protocol Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
Scan protocol Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm.
Submission date Oct 18, 2011
Last update date Jan 01, 2014
Contact name ROCIO BENITO
Organization name centro investigacion del cancer
Lab 12
Street address Campus Unamuno s/n
City Salamanca
ZIP/Postal code 37007
Country Spain
Platform ID GPL570
Series (1)
GSE33075 Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression

Data table header descriptions

Data table
AFFX-BioB-5_at 6.609282
AFFX-BioB-M_at 7.604414
AFFX-BioB-3_at 7.227222
AFFX-BioC-5_at 8.543254
AFFX-BioC-3_at 8.714776
AFFX-BioDn-5_at 9.418457
AFFX-BioDn-3_at 11.08167
AFFX-CreX-5_at 12.09652
AFFX-CreX-3_at 12.57497
AFFX-DapX-5_at 7.930833
AFFX-DapX-M_at 9.771966
AFFX-DapX-3_at 10.25171
AFFX-LysX-5_at 4.858616
AFFX-LysX-M_at 6.013255
AFFX-LysX-3_at 6.97601
AFFX-PheX-5_at 5.934622
AFFX-PheX-M_at 6.906443
AFFX-PheX-3_at 7.049184
AFFX-ThrX-5_at 6.272808
AFFX-ThrX-M_at 7.287455

Total number of rows: 54675

Table truncated, full table size 1051 Kbytes.

Supplementary file Size Download File type/resource
GSM818678_2623.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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