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Sample GSM818813 Query DataSets for GSM818813
Status Public on Jan 01, 2014
Title NOLEU_3178
Sample type RNA
 
Source name healthy donor
Organism Homo sapiens
Characteristics source: Bone Marrow
tissue type: healthy donor
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
Label biotin
Label protocol Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
 
Hybridization protocol Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
Scan protocol Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm
 
Submission date Oct 18, 2011
Last update date Jan 01, 2014
Contact name ROCIO BENITO
E-mail(s) beniroc@usal.es
Organization name centro investigacion del cancer
Lab 12
Street address Campus Unamuno s/n
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL570
Series (1)
GSE33075 Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression

Data table header descriptions
ID_REF
VALUE RMA NORMALIZED EXPRESSION VALUE

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.545236
AFFX-BioB-M_at 7.80231
AFFX-BioB-3_at 7.37367
AFFX-BioC-5_at 8.899401
AFFX-BioC-3_at 9.243759
AFFX-BioDn-5_at 9.412115
AFFX-BioDn-3_at 11.48019
AFFX-CreX-5_at 12.16027
AFFX-CreX-3_at 12.61362
AFFX-DapX-5_at 4.042174
AFFX-DapX-M_at 6.234399
AFFX-DapX-3_at 7.508513
AFFX-LysX-5_at 2.954026
AFFX-LysX-M_at 4.002162
AFFX-LysX-3_at 5.158615
AFFX-PheX-5_at 2.974755
AFFX-PheX-M_at 3.983387
AFFX-PheX-3_at 5.95962
AFFX-ThrX-5_at 3.250462
AFFX-ThrX-M_at 3.238818

Total number of rows: 54675

Table truncated, full table size 1052 Kbytes.




Supplementary file Size Download File type/resource
GSM818813_SAL_00142.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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