NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM818817 Query DataSets for GSM818817
Status Public on Jan 01, 2014
Title NOLEU_3557
Sample type RNA
 
Source name healthy donor
Organism Homo sapiens
Characteristics source: Bone Marrow
tissue type: healthy donor
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from purified cell populations using the RNeasy Mini Kit (Qiagen, Valencia, CA,USA) according to the manufacturer’s instructions. The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent,
Palo Alto, CA, USA).
Label biotin
Label protocol Labeling was performed according to protocols from Affymetrix. Briefly, 5000 ng of total RNA was amplified and labeled. These samples were fragmented and combined with array hybridization controls (Affymetrix, Santa Clara, CA, USA) in hybridization buffer. 11 µg of cRNA target were then hybridized with the GeneChip HG_U133 Plus 2.0 array (Affymetrix)
 
Hybridization protocol Hybridizations were performed according to protocols from Affymetrix.
Labeled RNA was hybridized to Human Genome U133 plus 2.0 microarray (Affymetrix).
Scan protocol Washing and scanning were performed using Fluidics Station 400 and
GeneChip Scanner (Affymetrix Inc.).
Data processing Expression value for each probe set was calculated using RMAExpress program that uses RMA (robust multi-array average) algorithm
 
Submission date Oct 18, 2011
Last update date Jan 01, 2014
Contact name ROCIO BENITO
E-mail(s) beniroc@usal.es
Organization name centro investigacion del cancer
Lab 12
Street address Campus Unamuno s/n
City Salamanca
ZIP/Postal code 37007
Country Spain
 
Platform ID GPL570
Series (1)
GSE33075 Imatinib therapy of chronic myeloid leukemia restores the expression levels of key genes for DNA damage and cell cycle progression

Data table header descriptions
ID_REF
VALUE RMA NORMALIZED EXPRESSION VALUE

Data table
ID_REF VALUE
AFFX-BioB-5_at 6.549109
AFFX-BioB-M_at 7.700157
AFFX-BioB-3_at 7.254378
AFFX-BioC-5_at 8.78533
AFFX-BioC-3_at 9.08469
AFFX-BioDn-5_at 9.321625
AFFX-BioDn-3_at 11.12162
AFFX-CreX-5_at 12.06369
AFFX-CreX-3_at 12.48638
AFFX-DapX-5_at 4.873907
AFFX-DapX-M_at 6.893034
AFFX-DapX-3_at 7.516759
AFFX-LysX-5_at 2.835654
AFFX-LysX-M_at 3.64745
AFFX-LysX-3_at 4.605071
AFFX-PheX-5_at 3.33146
AFFX-PheX-M_at 4.214502
AFFX-PheX-3_at 5.395992
AFFX-ThrX-5_at 3.524478
AFFX-ThrX-M_at 3.896349

Total number of rows: 54675

Table truncated, full table size 1051 Kbytes.




Supplementary file Size Download File type/resource
GSM818817_SAL_00200.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap