|
| Status |
Public on Nov 01, 2011 |
| Title |
Sox3 over-expression in neural progenitor cells, biol repl 2 |
| Sample type |
RNA |
| |
|
| Source name |
Neural progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: ES cell derived neural progenitor cells
genotype: Sox1-GFP and Nestin-Sox3
|
| Treatment protocol |
Cells were trypsinized and FACS sorted at day 4.
|
| Growth protocol |
Sox1-GFP or Sox1-GFP/Nestin-Sox3 positive mouse ES-cells were differentiated for 4 days with B27 and N2 supplement (GIBCO) as monolayers on gelatinized cellbinding surface (Corning) with 0.5uM retinoic acid (all-trans, Sigma) at day 0 and day 2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen mRNA preparation kit
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Sox3 over-expression
MB5_+Sox3_Sox1+NPC.CEL
|
| Data processing |
The data was processed with RMA using Affymetrix Power Tools and a custom CDF (Brainarray version 11.0.1, RefSeq).
|
| |
|
| Submission date |
Oct 18, 2011 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Rickard Sandberg |
| E-mail(s) |
Rickard.Sandberg@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of Cell and Molecular Biology
|
| Street address |
Berzelius vag 35
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL14757 |
| Series (2) |
| GSE33024 |
Sequentially acting Sox transcription factors in neural lineage development |
| GSE33061 |
Sequentially acting Sox transcription factors in neural lineage development [microarray] |
|
