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Sample GSM818960 Query DataSets for GSM818960
Status Public on Nov 01, 2011
Title Control neural progenitor cells, biol repl 1
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics cell type: ES cell derived neural progenitor cells
genotype: Sox1-GFP
Treatment protocol Cells were trypsinized and FACS sorted at day 4.
Growth protocol Sox1-GFP or Sox1-GFP/Nestin-Sox3 positive mouse ES-cells were differentiated for 4 days with B27 and N2 supplement (GIBCO) as monolayers on gelatinized cellbinding surface (Corning) with 0.5uM retinoic acid (all-trans, Sigma) at day 0 and day 2.
Extracted molecule total RNA
Extraction protocol Qiagen mRNA preparation kit
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description Control
MB1_ctrl_Sox1+NPC.CEL
Data processing The data was processed with RMA using Affymetrix Power Tools and a custom CDF (Brainarray version 11.0.1, RefSeq).
 
Submission date Oct 18, 2011
Last update date Nov 01, 2011
Contact name Rickard Sandberg
E-mail(s) Rickard.Sandberg@ki.se
Organization name Karolinska Institutet
Department Department of Cell and Molecular Biology
Street address Berzelius vag 35
City Stockholm
ZIP/Postal code 17177
Country Sweden
 
Platform ID GPL14757
Series (2)
GSE33024 Sequentially acting Sox transcription factors in neural lineage development
GSE33061 Sequentially acting Sox transcription factors in neural lineage development [microarray]

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 7.8949
AFFX-BioB-M_at 8.3475
AFFX-BioB-3_at 7.8115
AFFX-BioC-5_at 8.1539
AFFX-BioC-3_at 7.8311
AFFX-BioDn-5_at 10.7586
AFFX-BioDn-3_at 11.9594
AFFX-CreX-5_at 13.1491
AFFX-CreX-3_at 13.4284
AFFX-DapX-5_at 5.4099
AFFX-DapX-M_at 8.5951
AFFX-DapX-3_at 9.4367
AFFX-LysX-5_at 5.0398
AFFX-LysX-M_at 6.357
AFFX-LysX-3_at 7.3014
AFFX-PheX-5_at 5.3758
AFFX-PheX-M_at 6.0119
AFFX-PheX-3_at 7.3529
AFFX-ThrX-5_at 5.7981
AFFX-ThrX-M_at 5.8045

Total number of rows: 21940

Table truncated, full table size 444 Kbytes.




Supplementary file Size Download File type/resource
GSM818960.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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