|
| Status |
Public on Feb 04, 2006 |
| Title |
RNAi control replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Kc-167 cells control RNAi (beta-lactamase)
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Kc-167 cells, 5 day treatment with beta-lactamase specific RNAi. Specifics:1.6X10^6 cells in 0.5mL of serum-free Sang's M3 media incubated for 1 hour with 20ug of dsRNA, subsequently equal volume of M3 media containing 10% serum was added. Cells harvested after 5 days. RNAi efficiency measured by immunoblot.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Suspension growing cells were pelleted by centifugation and RNA extracted by TRIzol.
|
| Label |
biotin
|
| Label protocol |
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Kc-167 cells, 5 day treatment with beta-lactamase specific RNAi.
|
| Data processing |
gcRMA through R based affylmGUI module
|
| |
|
| Submission date |
Nov 04, 2005 |
| Last update date |
Nov 04, 2005 |
| Contact name |
Spyros Artavanis-Tsakonas |
| E-mail(s) |
tsakonas@helix.mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
MGH Cancer Research Center
|
| Lab |
Artavanis-Tsakonas
|
| Street address |
7308 Building 149, 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE3566 |
Enigma (CG9006) RNAi vs control RNAi in Drosophila Kc-167 cells. |
|
