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Sample GSM821062 Query DataSets for GSM821062
Status Public on Dec 09, 2011
Title mdm 325 Ttn+
Sample type RNA
 
Source name mouse 325 with Ttn-mutation
Organism Mus musculus
Characteristics strain: B6C3Fe a/a-Ttnmdm/J
genotype: Titin mutant
tissue: quadriceps
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse 325 with Titin-mutation
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of HGU133_2 -type from Affymetrix. A Custom CDF Version 11 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized using RMA.
 
Submission date Oct 21, 2011
Last update date Dec 10, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE33157 Sarcolipin is a potential biomarker for dystrophy and is involved in inflammatory processes in skeletal muscles

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.635611
AFFX-BioB-M_at 9.071947
AFFX-BioB-3_at 8.84999
AFFX-BioC-5_at 9.829536
AFFX-BioC-3_at 10.19382
AFFX-BioDn-5_at 11.35446
AFFX-BioDn-3_at 12.2758
AFFX-CreX-5_at 13.56728
AFFX-CreX-3_at 13.54393
AFFX-DapX-5_at 7.76974
AFFX-DapX-M_at 9.013374
AFFX-DapX-3_at 9.671817
AFFX-LysX-5_at 6.676359
AFFX-LysX-M_at 7.044721
AFFX-LysX-3_at 7.429701
AFFX-PheX-5_at 7.037206
AFFX-PheX-M_at 7.078554
AFFX-PheX-3_at 7.430511
AFFX-ThrX-5_at 6.945824
AFFX-ThrX-M_at 7.183628

Total number of rows: 16553

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM821062_325_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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