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Sample GSM821063 Query DataSets for GSM821063
Status Public on Dec 09, 2011
Title mdm 326 Ttn+
Sample type RNA
 
Source name mouse 326 with Ttn-mutation
Organism Mus musculus
Characteristics strain: B6C3Fe a/a-Ttnmdm/J
genotype: Titin mutant
tissue: quadriceps
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse 326 with Titin-mutation
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of HGU133_2 -type from Affymetrix. A Custom CDF Version 11 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized using RMA.
 
Submission date Oct 21, 2011
Last update date Dec 10, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE33157 Sarcolipin is a potential biomarker for dystrophy and is involved in inflammatory processes in skeletal muscles

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.566867
AFFX-BioB-M_at 8.974617
AFFX-BioB-3_at 8.731171
AFFX-BioC-5_at 9.758266
AFFX-BioC-3_at 10.12651
AFFX-BioDn-5_at 11.33428
AFFX-BioDn-3_at 12.30598
AFFX-CreX-5_at 13.52563
AFFX-CreX-3_at 13.50879
AFFX-DapX-5_at 8.392
AFFX-DapX-M_at 9.463032
AFFX-DapX-3_at 9.876298
AFFX-LysX-5_at 6.622564
AFFX-LysX-M_at 7.06259
AFFX-LysX-3_at 7.451597
AFFX-PheX-5_at 6.918151
AFFX-PheX-M_at 7.109864
AFFX-PheX-3_at 7.407767
AFFX-ThrX-5_at 7.219649
AFFX-ThrX-M_at 7.445856

Total number of rows: 16553

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM821063_326_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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