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Sample GSM821064 Query DataSets for GSM821064
Status Public on Dec 09, 2011
Title mdm 328 Ttn-
Sample type RNA
 
Source name mouse 328 without Ttn-mutation
Organism Mus musculus
Characteristics strain: B6C3Fe a/a-Ttnmdm/J
genotype: Titin wild type
tissue: quadriceps
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse 328 without Titin-mutation
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of HGU133_2 -type from Affymetrix. A Custom CDF Version 11 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized using RMA.
 
Submission date Oct 21, 2011
Last update date Dec 10, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE33157 Sarcolipin is a potential biomarker for dystrophy and is involved in inflammatory processes in skeletal muscles

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.83603
AFFX-BioB-M_at 9.174805
AFFX-BioB-3_at 8.944891
AFFX-BioC-5_at 9.952839
AFFX-BioC-3_at 10.30818
AFFX-BioDn-5_at 11.46298
AFFX-BioDn-3_at 12.35812
AFFX-CreX-5_at 13.54393
AFFX-CreX-3_at 13.52563
AFFX-DapX-5_at 8.462143
AFFX-DapX-M_at 9.506635
AFFX-DapX-3_at 9.991712
AFFX-LysX-5_at 6.751691
AFFX-LysX-M_at 7.192464
AFFX-LysX-3_at 7.658472
AFFX-PheX-5_at 7.078844
AFFX-PheX-M_at 7.20572
AFFX-PheX-3_at 7.61113
AFFX-ThrX-5_at 7.384045
AFFX-ThrX-M_at 7.524309

Total number of rows: 16553

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM821064_328_Mouse430_2_.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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