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Sample GSM821065 Query DataSets for GSM821065
Status Public on Dec 09, 2011
Title mdm 335 Ttn-
Sample type RNA
 
Source name mouse 335 without Ttn-mutation
Organism Mus musculus
Characteristics strain: B6C3Fe a/a-Ttnmdm/J
genotype: Titin wild type
tissue: quadriceps
Extracted molecule total RNA
Extraction protocol RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Description quadriceps RNA of mouse 335 without Titin-mutation
Data processing The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of HGU133_2 -type from Affymetrix. A Custom CDF Version 11 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized using RMA.
 
Submission date Oct 21, 2011
Last update date Dec 10, 2011
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE33157 Sarcolipin is a potential biomarker for dystrophy and is involved in inflammatory processes in skeletal muscles

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.81724
AFFX-BioB-M_at 9.157678
AFFX-BioB-3_at 8.997988
AFFX-BioC-5_at 9.992832
AFFX-BioC-3_at 10.33428
AFFX-BioDn-5_at 11.49141
AFFX-BioDn-3_at 12.30598
AFFX-CreX-5_at 13.52563
AFFX-CreX-3_at 13.48285
AFFX-DapX-5_at 8.322412
AFFX-DapX-M_at 9.464884
AFFX-DapX-3_at 9.863864
AFFX-LysX-5_at 6.742453
AFFX-LysX-M_at 7.106085
AFFX-LysX-3_at 7.644743
AFFX-PheX-5_at 7.09561
AFFX-PheX-M_at 7.215129
AFFX-PheX-3_at 7.599875
AFFX-ThrX-5_at 7.31629
AFFX-ThrX-M_at 7.459222

Total number of rows: 16553

Table truncated, full table size 295 Kbytes.




Supplementary file Size Download File type/resource
GSM821065_335_Mouse430_2_.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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