|
| Status |
Public on Oct 25, 2011 |
| Title |
AC-1 |
| Sample type |
RNA |
| |
|
| Source name |
ACC2 Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ACC2
cell type: Adenoid Cystic Carcinoma
|
| Growth protocol |
Both cell lines were cultured in DMEM supplemented with Fetal Bovine Serum 10% and Penicillin-Streptomycin 1% until confluence.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Culture medium was aspirated from plates. The plates were flushed with 1 ml of ISOGEN (Nippongene, Japan). The mix was collected and centrifuged. The fraction containing Total RNA was collected, the rest discarded. RNA was then decanted and concentrated to 1ɥg/ɥl. RNA was the checked for purity (Eukaryote Total RNA nano Series II, Agilent, USA).
|
| Label |
Biotin
|
| Label protocol |
RNA was labeled with the 3'-IVT Labeling Kit (Affymetrix) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
RNA hybridization was conducted with GeneAtlas Hybridization, Wash, and Stain Kit for 3' IVT Arrays (Affymetrix, USA) using the GeneAtlas System.
|
| Scan protocol |
Arrays were scanned by the GeneAtlas Imaging Station incorporated on the GeneAtlas System.
|
| Description |
SAMPLE 2
|
| Data processing |
Data was generated by the GeneAtlas Instrument Control Software and the Partek express software for statistical analysis. The data was the filtered using the GeneSpring GX software. The processing algorithm used is RMA
|
| |
|
| Submission date |
Oct 24, 2011 |
| Last update date |
Oct 25, 2011 |
| Contact name |
Alejandro Mayorca-Guiliani |
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
790-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE33171 |
Gene expression comparison between two human cancer cell Lines: Oral squamous cell carcinoma SASL1m and adenoid cystic carcinoma ACC2 |
|
