|
| Status |
Public on Dec 06, 2011 |
| Title |
PRB25850 |
| Sample type |
RNA |
| |
|
| Source name |
Castration
|
| Organism |
Homo sapiens |
| Characteristics |
strain: NOD/SCID
tumor type: LuCaP35
disease type: Prostate cancer
|
| Treatment protocol |
Surgical castration or sham control were performed on mice with matched tumor sizes. Tumors were harvested 4 weeks post castration.
|
| Growth protocol |
The LuCaP35 tumors were inoculated subcutaneously in NOD/SCID mice, and grew for ~1 month.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy kit used for the extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Genome U133P 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Oct 28, 2011 |
| Last update date |
Dec 06, 2011 |
| Contact name |
Yuting Sun |
| Organization name |
Genentech
|
| Department |
Research Oncology
|
| Street address |
1 DNA way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE33316 |
The effect of androgen deprivation on human prostate xenograft tumor LuCaP35 |
|
