|
| Status |
Public on Sep 01, 2012 |
| Title |
TSC22D4 Knockdown Mouse Liver 3 + 4 (pool) |
| Sample type |
RNA |
| |
|
| Source name |
Liver, excized after 1 week of knockdown
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BI/6
genotype/variation: Adenovirus mediated shRNA knockdown of TSC22D4
tissue: Liver, excized after 1 week of knockdown
|
| Treatment protocol |
Adenovirus mediated shRNA knockdown of TSC22D4 in the liver
|
| Growth protocol |
Mice were fed ad libitum and sacrificed in the morning.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Quiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
TSC_B
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 12 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Oct 28, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9746 |
| Series (1) |
| GSE33324 |
Cachexia-inducible Transforming growth factor-beta1 stimulated Clone-22 D4 controls acute hepatic lipid homeostasis. |
|
