|
| Status |
Public on Nov 05, 2011 |
| Title |
Heart 2 month old Taz KD rep1 (mouse 5972) |
| Sample type |
RNA |
| |
|
| Source name |
Heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: transgenic shRNA Taz KD
genetic background: C57BL/6J
tissue: heart
age: 2 mo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol folowed by Dnase treatment using Qiagen Rneasy minikit using manufacturers protocls. Quality control was performed with Agilent 2100 Expert Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRMA were prepared with the Ambion MessageAMP kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hydridization protocols
|
| Scan protocol |
Standard Illumina scanning protocols
|
| Description |
Taz shRNA KD replicate 1 625 mg/Kg Doxycycline diet
|
| Data processing |
The data were normalized using quantile normalization
|
| |
|
| Submission date |
Nov 03, 2011 |
| Last update date |
Nov 05, 2011 |
| Contact name |
Michael Andrew Kiebish |
| E-mail(s) |
kiebish@wustl.edu
|
| Phone |
314-362-2229
|
| Fax |
314-362-1402
|
| Organization name |
Washington University School of Medicine
|
| Department |
Internal Medicine
|
| Lab |
Richard W. Gross
|
| Street address |
660 South Euclid Avenue, Campus Box 8020
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE33452 |
Genome-wide analysis of gene transcription in myocardium of the doxycycline inducible Tafazzin shRNA knockdown mouse model of Barth syndrome |
|
