|
| Status |
Public on Jul 23, 2012 |
| Title |
wild-type mouse exposed to cigarette smoke, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Lung_wild-type_cigarette smoke exposure
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57Bl/6
genotype/variation: wild type
gender: male
age: 12 weeks
tissue: whole lung
|
| Treatment protocol |
Cigarette smoke exposure was performed in a stainless steel chamber (71 cm x 61 cm x 61 cm) using a smoking machine (Model TE-10, Teague Enterprises). The smoking machine puffs each 1R3F cigarette for 2 seconds, for a total of 9 puffs prior to ejection, at a flow rate of 1.05 l/min, providing a standard puff of 35 cm3. The smoke machine was adjusted to deliver 5 cigarettes at one time. Mice were exposed 5 days/week for 4 months.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 100ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 4X44K whole mouse gene expression array (014868). Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44K array slides
|
| Description |
smoke_WT_2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.3.1 (Agilent) using default parameters.
|
| |
|
| Submission date |
Nov 07, 2011 |
| Last update date |
Jul 23, 2012 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
panditkusum@yahoo.com
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE33512 |
Gene expression data on lungs of wild-type and Rora (Retinoic acid related orphan receptor) mutant mice exposed to room air and smoke |
|
