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Status
Public on Mar 01, 2006
Title
Saline T00278943
Sample type
RNA
Source name
liver
Organism
Mus musculus Characteristics
Male C57Bl/6 Mice were 10.5 weeks old at time of sacrifice.
Treatment protocol
Mice injected i.p. 2x/week for 4 weeks with saline, sacrificed 2 days after last dose.
Extracted molecule
total RNA
Extraction protocol
For RNA isolation, liver was homogenized in RNeasy RLT buffer using Polytron homogenizer, layered onto 5.7M CsCl2 and pelleted after overnight centrifugation at 35000 rpm, room temp. The pellet was resuspended in RLT buffer and RNA was isolated on RNeasy midi columns following the manufacturer's instructions, and including an on-column DNase digestion step.
Label
biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
Control group
Data processing
CodeLink Expression Analysis 4.1.0.29054
Submission date
Nov 11, 2005
Last update date
Nov 14, 2005
Contact name
Michael Pear
E-mail(s)
mpear@isisph.com Phone
760-603-2779
URL
http://www.isspharm.com Organization name
Isis Pharmaceuticals, Inc.
Department
Research Informatics
Street address
1896 Rutherford Rd
City
Carlsbad
State/province
CA
ZIP/Postal code
92009
Country
USA
Platform ID
GPL2897 Series (1)
GSE3603 Microarray analysis of in vivo inhibition of mir-122 with an antisense oligonucleotide.
Data table header descriptions ID_REF VALUE Intensity normalized by median of spot-based raw intensity
RAW_INTENSITY Raw signal intensities corrected for background
QUALITY Numerical Quality score from codes G,S (Good, Saturated) =>1, L (near background) => .5, I, C, CI, CL (Ireggular, Background Contaminated) => 0
Data table ID_REF VALUE RAW_INTENSITY QUALITY 1001
104.8433556
3558.222168
1002
2.865708476
97.25773621
1
1003
0.414003339
14.05063629
1
1004
1.150203827
39.03614807
1
1005
0.629202483
21.35416412
1
1006
0.74565696
25.30644989
1
1007
122.5503961
4159.171875
1008
125.8840378
4272.310547
1010
1.751987801
59.4597702
1
1012
0.64476567
21.88235474
1
1013
0.95176943
32.30158997
1
1014
128.5767131
4363.695801
1015
143.5320905
4871.258301
1017
0.206255668
7
0.5
1018
0.45081605
15.30000305
1
1019
0.369950692
12.55555725
1
1020
0.10027646
3.40322876
0.5
1021
145.1243855
4925.29834
1022
118.9153845
4035.805176
1024
0.310907651
10.55172729
0.5
36227 1146 Kbytes .Supplementary data files not provided
