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Status
Public on Mar 01, 2006
Title
Saline T00278946
Sample type
RNA
Source name
liver
Organism
Mus musculus Characteristics
Male C57Bl/6 Mice were 10.5 weeks old at time of sacrifice.
Treatment protocol
Mice injected i.p. 2x/week for 4 weeks with saline, sacrificed 2 days after last dose.
Extracted molecule
total RNA
Extraction protocol
For RNA isolation, liver was homogenized in RNeasy RLT buffer using Polytron homogenizer, layered onto 5.7M CsCl2 and pelleted after overnight centrifugation at 35000 rpm, room temp. The pellet was resuspended in RLT buffer and RNA was isolated on RNeasy midi columns following the manufacturer's instructions, and including an on-column DNase digestion step.
Label
biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
Control group
Data processing
CodeLink Expression Analysis 4.1.0.29054
Submission date
Nov 11, 2005
Last update date
Nov 14, 2005
Contact name
Michael Pear
E-mail(s)
mpear@isisph.com Phone
760-603-2779
URL
http://www.isspharm.com Organization name
Isis Pharmaceuticals, Inc.
Department
Research Informatics
Street address
1896 Rutherford Rd
City
Carlsbad
State/province
CA
ZIP/Postal code
92009
Country
USA
Platform ID
GPL2897 Series (1)
GSE3603 Microarray analysis of in vivo inhibition of mir-122 with an antisense oligonucleotide.
Data table header descriptions ID_REF VALUE Intensity normalized by median of spot-based raw intensity
RAW_INTENSITY Raw signal intensities corrected for background
QUALITY Numerical Quality score from codes G,S (Good, Saturated) =>1, L (near background) => .5, I, C, CI, CL (Ireggular, Background Contaminated) => 0
Data table ID_REF VALUE RAW_INTENSITY QUALITY 1001
53.71870949
1160.870361
1002
2.215463578
47.87654114
1
1003
0.265705274
5.74193573
0.5
1004
1.241390961
26.82666779
1
1005
0.40579201
8.769233704
0.5
1006
0.652766049
14.10638428
1
1007
91.14235683
1969.601685
1008
109.7136256
2370.929932
1010
0.351082864
7.586959839
0.5
1012
0.747002988
16.14286041
1
1013
0.835774415
18.06122589
1
1014
69.9271538
1511.137573
1015
108.7951624
2351.081787
1017
0.209887915
4.535713196
0.5
1018
0.232774861
5.030303955
0.5
1019
0.267197955
5.77419281
0.5
1020
0.266078444
5.75
0.5
1021
93.41210695
2018.651367
1022
100.9066912
2180.610596
1024
0.204421343
4.417579651
0.5
36227 1150 Kbytes .Supplementary data files not provided
