|
| Status |
Public on Nov 26, 2019 |
| Title |
PRMT6_shRNA1_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
cell line: CCE
shRNA: PRMT6
|
| Treatment protocol |
shRNA (Prmt6 or GFP) cloned into pSuper.puro (Oligoengine) were transfected into CCE using Lipofectamine. 18h later, puromycin (1.5ug/ml) containing media was changed and selected for 4days.
|
| Growth protocol |
CCE (Stem Cell Tech) ES cells, maintained feeder-free, were used for transient shRNA knockdown in DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 1000 / ml of LIF (Chemicon).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Data was VST transformed, RSN normalized in R using LUMI package
|
| |
|
| Submission date |
Nov 11, 2011 |
| Last update date |
Nov 26, 2019 |
| Contact name |
Yen-Sin Ang |
| Organization name |
Gladstone Institute, UCSF
|
| Street address |
1650 Owens Street
|
| City |
san francisco |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6481 |
| Series (1) |
| GSE33635 |
Prmt6 is an essential regulator of pluripotency and reprogramming |
|
