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Status |
Public on Nov 26, 2019 |
Title |
PRMT6_shRNA2_replicate1 |
Sample type |
RNA |
|
|
Source name |
Embryonic Stem Cell
|
Organism |
Mus musculus |
Characteristics |
cell line: CCE shRNA: PRMT6
|
Treatment protocol |
shRNA (Prmt6 or GFP) cloned into pSuper.puro (Oligoengine) were transfected into CCE using Lipofectamine. 18h later, puromycin (1.5ug/ml) containing media was changed and selected for 4days.
|
Growth protocol |
CCE (Stem Cell Tech) ES cells, maintained feeder-free, were used for transient shRNA knockdown in DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 1000 / ml of LIF (Chemicon).
|
Extracted molecule |
total RNA |
Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
Data was VST transformed, RSN normalized in R using LUMI package
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|
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Submission date |
Nov 11, 2011 |
Last update date |
Nov 26, 2019 |
Contact name |
Yen-Sin Ang |
Organization name |
Gladstone Institute, UCSF
|
Street address |
1650 Owens Street
|
City |
san francisco |
State/province |
CALIFORNIA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL6481 |
Series (1) |
GSE33635 |
Prmt6 is an essential regulator of pluripotency and reprogramming |
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