|
| Status |
Public on Nov 14, 2012 |
| Title |
wtbrain-VSV-2d-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
brain
|
| Organism |
Mus musculus |
| Characteristics |
age: 2 months
strain: C57BL/6
genotype: wt
time point: 2 days
agent: vesicular stomatitis virus (VSV)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed DNase I treatment and clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
biotinylated cRNAs prepared using Ambion’s Illumina TotalPrep-96 RNA Amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
2 days after intrasal VSV infection
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI
|
| |
|
| Submission date |
Nov 14, 2011 |
| Last update date |
Nov 14, 2012 |
| Contact name |
Ganes C. Sen |
| E-mail(s) |
seng@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Street address |
9500 Euclid Avenue NE-20
|
| City |
Cleveland |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE33678 |
Gene expression profile changes in brains of VSV-infected wild-type and Ifit2 knockout mice at 6 days after infection |
|
