|
Status |
Public on Jan 23, 2020 |
Title |
FGF21 KO, WAT, DIO, biological replicate 2 |
Sample type |
RNA |
|
|
Source name |
Epididymal white adipose tissue
|
Organism |
Mus musculus |
Characteristics |
strain: FGF21 KO c57b6/sv129 mixed bkg age: 4-5 months old treatment: High Fat diet for 10 weeks genetic background: mixed c57b6/sv129 tissue: Epididymal white adipose tissue disease state: Diet-induced Obesity
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
The data were normalized using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
|
|
Submission date |
Nov 14, 2011 |
Last update date |
Jan 23, 2020 |
Contact name |
Ruth T Yu |
Organization name |
Salk Institute
|
Department |
Gene Expression Lab
|
Lab |
Ronald Evans
|
Street address |
10010 N Torrey Pines Rd
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE33684 |
Fibroblast Growth Factor-21 Regulates PPARĪ³ Activity and the Antidiabetic Actions of Thiazolidinediones |
|