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Sample GSM832942 Query DataSets for GSM832942
Status Public on Dec 15, 2011
Title D2_Erythrocytes_(Ter119+)_rep3
Sample type RNA
 
Source name primary bone marrow
Organism Mus musculus
Characteristics strain: DBA2
cell type: Erythrocytes_(Ter119+)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the miRNeasy Mini Kit (Qiagen) in accordance with the manufacturer’s protocol.
Label Cy3
Label protocol 100 ng of total RNA was labeled and hybridized to Agilent 8x15K Mouse miRNA arrays, based on version 11 release of Sanger Inst miRBASE. Labeling, hybridization and washing were performed by Oxford Gene Technology using the Agilent Mouse microRNA Microarray Kit.
 
Hybridization protocol Labeling, hybridization and washing were performed by Oxford Gene Technology using the Agilent Mouse microRNA Microarray Kit.
Scan protocol Slides were scanned at 5 μm resolution utilizing the Agilent eXtended Dynamic range function combining data from scans at 5% and 100% PMT power.
Data processing Data were extracted using Agilent Feature Extraction Software version 9.5.3.1 and thresholded at 1, log2-transformed and normalized to the 75th percentile using GeneSpring GX11.0 (Agilent).
 
Submission date Nov 14, 2011
Last update date Dec 15, 2011
Contact name Leonid Bystrykh
E-mail(s) l.bystrykh@umcg.nl
Organization name University Medical Center Groningen
Department European Research Institute for the Biology of Ageing - ERIBA
Lab Ageing Biology and Stem Cells
Street address A. Deusinglaan 1
City Groningen
State/province Groningen
ZIP/Postal code 9713 AV
Country Netherlands
 
Platform ID GPL8824
Series (2)
GSE33690 MicroRNA profiles of four developmentally related hematopoietic cell types isolated from the BM of B6 and D2 mice.
GSE33691 Genetic Screen Identifies MicroRNA Cluster 99b/let-7e/125a as a Regulator of Primitive Hematopoietic Cells

Data table header descriptions
ID_REF
VALUE GeneSpring log2-transformed and 75th percentile normalized signal intensity
DETECTION_CALL

Data table
ID_REF VALUE DETECTION_CALL
mghv-miR-M1-1 -2.95 A
mghv-miR-M1-2 1.28 P
mghv-miR-M1-3 -2.95 A
mghv-miR-M1-4 -2.95 A
mghv-miR-M1-5 -2.95 A
mghv-miR-M1-6 -2.95 A
mghv-miR-M1-7-3p -0.30 A
mghv-miR-M1-7-5p 0.93 P
mghv-miR-M1-8 -2.33 A
mghv-miR-M1-9 -1.18 A
mmu-let-7a 7.12 P
mmu-let-7b 4.32 P
mmu-let-7b* 1.36 P
mmu-let-7c 5.28 P
mmu-let-7c-1* -0.42 A
mmu-let-7c-2* -2.37 A
mmu-let-7d 6.40 P
mmu-let-7d* -0.93 A
mmu-let-7e -2.95 A
mmu-let-7f 7.04 P

Total number of rows: 577

Table truncated, full table size 11 Kbytes.




Supplementary file Size Download File type/resource
GSM832942.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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